| 摘要 |
A method is described for detecting analytes which specifically bind to a specific binding molecule using proximity modulated signal generation. The method uses a first construct which includes a specific binding moiety, such as an antibody, attached to a photosensitizer moiety, and a second construct which includes a second binding molecule which recognizes the bound analyte and which also contains a photocleavable linker linked to an amplifiable nucleic acid oligomer. Upon exposure to activating light, the photosensitizer generates singlet oxygen causing cleavage of the photocleavable linker in analyte-bound second constructs and releasing the corresponding amplifiable oligomer. Following amplification, the amplified oligomer can be detected and/or quantitated in conventional ways, thereby identifying the corresponding analyte.
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