发明名称 METHOD FOR IMPROVED TRANSFER OF DNA INTO LIVING CELLS BY ELECTROPORATION
摘要 The subject invention concerns an electroporation buffer that allows for enhanced transfection efficiency and cell viability of cells during application of an electric current. Buffers of the invention provide for maximum transfer of target particles into cells while maintaining the health and growth potential of the cell population. Compositions of the invention comprise electroporation buffers of approximately physiological ionic strength and pH, and having serum or purified proteins, such as serum albumin, added thereto. The subject invention is suitable for use with any cell type. The subject invention also concerns methods of electroporation using an electroporation buffer of the invention.
申请公布号 US2009197335(A1) 申请公布日期 2009.08.06
申请号 US20090364409 申请日期 2009.02.02
申请人 MOFFITT CANCER CENTER 发明人 AGRAWAL DEEPAK KUMAR;PLEDGER WARREN JACKSON;KLUFT JONATHAN A.;AGRAWAL ASHA
分类号 C12N15/01;C12N5/00 主分类号 C12N15/01
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