METHOD, PROCESS, AND KIT FOR DIAGNOSIS OR PROGNOSIS OF COLORECTAL CANCER

摘要

The invention relates to a method or a process and a kit for the diagnosis/prognosis of colorectal cancer, that comprises the following steps: a. extracting a nucleic material of a biological sample; b. using at least one pair of amplification primers for obtaining amplicons of at least one target sequence; c. using at least one detection probe for detecting the presence of said amplicons; characterised in that said method includes a modification step between steps a) and b). Advantageously, steps b) and c) are carried out simultaneously. More precisely, the target sequence is included in gene WIF1, and said primer pair includes at least one amplification primer including at least 15 nucleotide patterns of a nucleotide sequence selected from SEQ ID n° 1 to 10 and/or said detection probe includes at least 15 nucleotide patterns of a nucleotide sequence selected from SEQ ID n° 1 to 10.