发明名称 |
Reverse genetics of negative-strand rna viruses in yeast |
摘要 |
The present invention relates to a methodology for the generation of infectious ribonucleoparticles (RNPs) of negative-strand RNA viruses, and in particular of non-segmented negative-strand RNA viruses in yeast, especially in budding yeast. Accordingly, the patent application relates to a recombinant yeast strain suitable for the rescue of infectious non-segmented negative-strand RNA virus particles or infectious virus-like particles. The invention also relates to the use of the recombinant yeast to prepare vaccine seed and to the use of the produced RNPs or RNPs-like to prepare vaccine formulations. It also concerns the use of the recombinant yeast for the screening of libraries of DNA. |
申请公布号 |
EP2085479(A1) |
申请公布日期 |
2009.08.05 |
申请号 |
EP20080290087 |
申请日期 |
2008.01.31 |
申请人 |
INSTITUT PASTEUR;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE |
发明人 |
MILED, CHAOUKI;TANGY, FREDERIC;JACOB, YVES |
分类号 |
C12N15/81;C12N7/02;C12N7/04 |
主分类号 |
C12N15/81 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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