PCR COMPOSITION COMPRISING ETHYLENE GLYCOL TETRAACETIC ACID AS A CHELATING AGENT FOR REDUCING OR PREVENTING PCR INHIBITION AND NON-SPECIFIC AMPLIFICATION CAUSED BY METAL ION

摘要

A composition for PCR, which contains an ethyleneglycoltetraacetic acid as a chelating agent is provided to enhance the PCR amplification efficiency and minimize the effect on concentration of ion ingredients. A composition for PCR comprises ethyleneglycoltetraacetic acid or its derivative as a chelating agent. The ethyleneglycoltetraacetic acid derivative is 1,2-bis(2-aminophenoxy)ethan-N,N,N',N'-tetraactic acid (BAPTA) or nitropheny-EGTA. The EGTA or its derivative is contained in the concentration of 0.001-10 mM. The composition for PCR is comprised in a kit for gene amplification and kit for diagnosing diseases.