METHOD OF MANUFACTURING INDUCED PLURIPOTENT STEM CELL ORIGINATED FROM SOMATIC CELL

摘要

A method for producing a pluripotent stem cell which is derived from a somatic cell is provided to have excellent production efficiency and stably express the differentiation function of the stem cell. A method for manufacturing a stem cell comprises: a step of preparing Oct4 gene, Sox2 gene, C-myc gene, and Klf4 gene from a mouse embryonic stem cell; a step of infecting each gene to a host cell using a lentiviral vector system to produce a gene induced virus; a step of mixing the virus concentrated mixture with a fist culturing; a step of mixing the somatic cell and virus solution to prepare somatic cell-virus mixture solution; a step of adding and maintaining a second culture solution; and a step of culturing a culture medium.