Cellular permissivity factor for viruses, and uses thereof

摘要

Disclosed is a method of facilitating production of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) from a culture of vertebrate cells, comprising the steps of: (a) providing a recombinant vertebrate cell transfected with an exogenous polynucleotide that encodes a mammalian CD163 polypeptide, so that expression of CD163 polypeptide in said cell is increased; (b) contacting a culture of said cell with PRRSV virus under conditions which permit infection of the cells and growth of the virus; and (c) recovering virus from said culture.