| 摘要 |
PROBLEM TO BE SOLVED: To provide an improved type humanized immunoglobulin which is substantially nonimmunogenic to human, easily and economically producible in a form suitable for application to a therapeutic agent, etc. , and to provide a therapeutic composition for treatment using the same. SOLUTION: The invention relates to a method for preparation of the humanized immunoglobulin exhibiting a binding affinity to the antigen of about 10<SP>8</SP>M<SP>-1</SP>or stronger, and having framework regions originating from light and heavy chains of human receptor immunoglobulins and complementarity determining regions (CDR), defined by Kabat, et al., from a donor immunoglobulin. The method includes steps of substituting at least one non CDR framework amino acids of the acceptor immunoglobulin with a corresponding amino acid originating from the donor immunoglobulin at a position in the immunoglobulins. COPYRIGHT: (C)2009,JPO&INPIT
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