METHOD FOR DETECTING PHOSPHOPANTETHEINYL TRANSFERASE ACTIVITY

摘要

The invention provides a method for detecting 4 -phosphopantetheinyl transferase (4 PPTase) activity, the method comprising the steps of: (a) immobilising a carrier protein domain or a functional fragment thereof on a solid support suitable for this purpose; (b) reacting the immobilised carrier protein domain or functional fragment thereof of step (a) with labelled coenzyme A and 4' PPTase to form a labelled carrier protein or functional fragment thereof; and (c) detecting the presence of the labelled carrier protein domain or functional fragment thereof.