RAPID MICROFLUIDIC THERMAL CYCLER FOR NUCLEIC ACID AMPLIFICATION

摘要

A system (100) for thermal cycling a material (115) to be thermal cycled including a microfluidic heat exchanger (101); a porous medium (113) in the microfluidic heat exchanger (101); a microfluidic thermal cycling chamber (103) containing the material (115) to be thermal cycled, the microfluidic thermal cycling chamber (103) operatively connected to the microfluidic heat exchanger (101); a working fluid at first temperature; a first system for transmitting the working fluid at first temperature to the microfluidic heat exchanger (101); a working fluid (104) at a second temperature, a second system for transmitting the working fluid (104) at second temperature to the microfluidic heat exchanger (101),- a pump (110) for flowing the working fluid (102) at the first temperature from the first system to the microfluidic heat exchanger (101) and through the porous medium (113); and flowing the working fluid (104) at the second temperature from the second system to the microfluidic heat exchanger (101) and through the porous medium (113).