| 摘要 |
FIELD: medicine. ^ SUBSTANCE: way of spinochrome A reception and the sea urchins protein interacting with polyhydroxynaphtoquinone is offered, characterised that raw materials are wash with water, extracted using ethyl alcohol, demineralised with 20% phosphoric acid; then the water-acid admixture is filtered, centrifugated, further the received solution is placed on polychrome 1, admixtures are washed out with 3% phosphoric acid and the distilled water, and the complex of protein with polyhydroxynaphtoquinone is eluted by 10% ethyl alcohol, then 40% ethyl alcohol, fractions are aggregated; further ethyl alcohol is removed, the water residue is freeze dried; received lyophilic powder is dissolved in the water, a non-dissolved deposit is separated, and ethyl alcohol is added to an aqueous solution at a parity 1:9 and maintained within 18-24 hours at 4C, the dropped out protein is separated, ethyl alcohol is washed out and chromatographed on the sephacryl S-200 and freeze dried; then spirituous solutions is aggregated, concentrated to dryness, the dry rest is dissolved in ethyl alcohol and chromatographed using sephadex LH-20, then the violet fraction of spinochrome A is separated, boiled to dryness, the dry residue is dissolved in acetone and crystallised. ^ EFFECT: expansion of a spectrum of biologically active substances received from sea urchins. ^ 1 ex, 3 dwg |
