RECOMBINANT EXPRESSION PLASMID VECTOR AND RECOMBINANT STRAIN TO BE USED IN PRODUCING OXALATE DECARBOXYLASE, AND METHOD OF PRODUCING RECOMBINANT OXALATE DECARBOXYLASE

摘要

It is intended to provide a means of highly producing oxalate decarboxylase originating in a microorganism. A recombinant expression plasmid vector, which contains a-amylase promoter of a microorganism belonging to the strain Bacillus and an oxalate decarboxylase gene originating in a microorganism that is provided under the control of the promoter, is constructed. A host bacterium is transformed by this vector to give an oxalate decarboxylase-producing bacterium. A recombinant oxalate decarboxylase is produced by culturing the producing bacterium as described above and then harvesting the oxalate decarboxylase thus produced.