| 摘要 |
An object of the present invention is to provide a primer capable of detecting a mycoplasma A. laidlawii (Acholeplasma laidlawii) sensitively and specifically. In order to achieve this object, the primer of the present invention is a primer for detecting A. laidlawii and for amplifying at least either one of polynucleotides containing the following nucleotide sequences (A) and (B) or partial sequences thereof: (A) a nucleotide sequence from position 589 to position 987 of SEQ ID NO: 1 or a sequence complementary thereto; (B) a nucleotide sequence from position 604 to position 975 of SEQ ID NO: 1 or a sequence complementary thereto. First, the region (A) is amplified and then the region (B) is amplified for the amplified product (A). Thus, A. laidlawii can be detected more sensitively and specifically.
|
