<p>The inventions is based on an expression enhancer sequence derived from the RNA-2 genome segment of a bipartite RNA virus, in which a target initiation site in the RNA-2 genome segment has been mutated. Deletion of appropriate start codons upstream of the main RNA2 translation initiation can greatly increase in foreign protein accumulation without the need for viral replication. Also provided are methods, vectors and systems, including the 'hyper-translatable' Cowpea Mosaic Virus ('CPMV-HT') based protein expression system.</p>
申请公布号
WO2009087391(A1)
申请公布日期
2009.07.16
申请号
WO2009GB00060
申请日期
2009.01.08
申请人
PLANT BIOSCIENCE LIMITED;SAINSBURY, FRANK;LOMONOSSOFF, GEORGE, PETER