摘要 |
Disclosed herein are methods and compositions related to engineered fragments of the human transglutaminase-related protein family, described herein as engineered transglutaminase barrel proteins (ETBPs), that have utility as high affinity, high selectivity target-binding proteins offering advantages as antibody equivalents for therapeutic, analytical, manufacturing and research purposes. ETBPs differ from naturally occurring human transglutaminase fragments by the addition, deletion, replacement and/or substitution of the naturally occurring amino acid sequence. ETBPs can be easily expressed in prokaryotic cells and in many cases can be purified by a simple solubilization and precipitation method. |
申请人 |
THE GENERAL HOSPITAL CORPORATION;GETMANOVA, ELENA, V.;KOVTUN, ALEXANDER;SUN, LIN;FRITSCH, EDWARD;SEED, BRIAN |
发明人 |
GETMANOVA, ELENA, V.;KOVTUN, ALEXANDER;SUN, LIN;FRITSCH, EDWARD;SEED, BRIAN |