发明名称 |
METHOD FOR PRODUCING TARGET PROTEINS USING AMINO ACIDS AND PYRUVIC ACIDS IN CULTURE OF PLANT CELLS |
摘要 |
Provided is a method for producing a target protein via cultivation of transgenic plant cells comprising a promoter capable of expressing the protein under sugar-free conditions or in response to the depletion of sugar and a gene encoding the target protein, without exchange of a cell growth medium with a sugar-depleted medium. The method comprises 1) culturing transgenic plant cells in a sugar-rich medium to grow plant cells; and 2) culturing the transgenic plant cells with addition of an amino acid mixture to the culture of Step 1 without exchange of a cell growth medium with a sugar-depleted medium, thereby expressing a target protein. The method of the present invention enables commercial-scale production of recombinant proteins via establishment of optimized culture conditions by addition of an amino acid mixture to induce protein expression without exchange of a cell growth medium with a sugar-free medium, and addition of pyruvic acid during the induction period of protein expression to thereby enhance the production yield of target proteins.
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申请公布号 |
US2009181428(A1) |
申请公布日期 |
2009.07.16 |
申请号 |
US20070160049 |
申请日期 |
2007.09.19 |
申请人 |
BORYUNG PHARMACEUTICAL CO., LTD. |
发明人 |
KIM SANG-LIN;TAN HYUN-KWANG;LIM SANG-MIN;RYU WUK-SANG;JUNG HAHN-SUN;LEE SONG-JAE;PARK CHEON-IK;KANG SEUNG-HOON;KIM DONG-IL |
分类号 |
C12P21/00 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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