CELL LINES FOR EXPRESSING ENZYME USEFUL IN THE PREPARATION OF AMIDATED PRODUCTS
摘要
Cell lines are provided for expressing peptidylglycine alpha-amidating monooxygenase (PAM), or one of its two catalytic domains. High levels of enzyme expression are achieved while utilizing a non-animal source, low protein tissue culture medium. A robust two-step downstream purification results in high enzyme purity. Resulting PAM, or its PHM catalytic domain, is used to catalyze the enzymatic conversion of X-Gly to X-alpha-hydroxy-Gly or X—NH2 (X being a peptide or any chemical compound having a carbonyl group to which a glycine group can be covalently attached). Methods of preparing preferred cell lines are also set forth.
申请公布号
EP1907561(A4)
申请公布日期
2009.07.15
申请号
EP20060785535
申请日期
2006.06.23
申请人
UNIGENE LABORATORIES, INC.
发明人
MILLER, DUNCAN, A.;MEHTA, NOZER, M.;CONSALVO, ANGELO, P.