| 摘要 |
The invention relates to methods of manipulating immobilised carbohydrates by derivatisation. Depending on the nature of the derivatisation, the carbohydrate may thereby be more easily detected and/or identified or handled. In particular, the invention relates to methods of preparing a reactive sugar comprising the steps of: i) providing a sample comprising a reducing sugar; ii) providing a solid support covalently attached to a linker comprising a capture group comprising an—NH2 group, wherein said linker optionally is attached to said solid support via a spacer; iii) reacting said reducing sugar with said—NH2 group, thereby obtaining an immobilised sugar; iv) reacting free—NH2 groups with a capping agent, wherein the capping agent comprises a reactive group capable of reacting with an—NH2 group; and v) reducing C═N bonds with a reducing agent, thereby obtaining an reactive sugar of the structure SugarCHn-NH—linked to a solid support via a linker and optionally a spacer, wherein n is 1 or 2. |
