Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.
申请公布号
WO2009085953(A2)
申请公布日期
2009.07.09
申请号
WO2008US87429
申请日期
2008.12.18
申请人
E. I. DU PONT DE NEMOURS AND COMPANY;LI, YOUGEN;NELSON, MARK, J.;LIAO, DER-ING;O'KEEFE, DANIEL, P.
发明人
LI, YOUGEN;NELSON, MARK, J.;LIAO, DER-ING;O'KEEFE, DANIEL, P.
