发明名称 |
SILENCING OF POLO-LIKE KINASE EXPRESSION USING INTERFERING RNA |
摘要 |
The present invention provides compositions comprising interfering RNA (e.g., siRNA, aiRNA, miRNA) that target polo-like kinase 1 (PLK-1) expression and methods of using such compositions to silence PLK-1 expression. More particularly, the present invention provides unmodified and chemically modified interfering RNA molecules which silence PLK-1 expression and methods of use thereof. The present invention also provides serum-stable nucleic acid-lipid particles (e.g., SNALP) comprising an interfering RNA molecule described herein, a cationic lipid, and a non-cationic lipid, which can further comprise a conjugated lipid that inhibits aggregation of particles. The present invention further provides methods of silencing PLK-1 gene expression by administering an interfering RNA molecule described herein to a mammalian subject. The present invention additionally provides methods of identifying and/or modifying PLK-1 interfering RNA having immunostimulatory properties. Methods for sensitizing a cell such as a cancer cell to the effects of a chemotherapy drug comprising sequentially delivering PLK-1 interfering RNA followed by the chemotherapy drug are also provided. |
申请公布号 |
WO2009082817(A1) |
申请公布日期 |
2009.07.09 |
申请号 |
WO2008CA02285 |
申请日期 |
2008.12.23 |
申请人 |
PROTIVA BIOTHERAPEUTICS, INC.;MACLACHLAN, IAN;JUDGE, ADAM |
发明人 |
MACLACHLAN, IAN;JUDGE, ADAM |
分类号 |
A61K9/00;A61K31/7105;A61K31/712;A61K31/713;A61K47/24;A61K47/28;A61K48/00;A61P35/00;C07H21/02;C12N9/12;C12N15/10;C12N15/113;C12N15/54;C12N15/88 |
主分类号 |
A61K9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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