| 摘要 |
<p>The present invention is related to a method for determining the sensitizing potential of a chemical (test) compound, comprising the steps of:
(a) Providing a suitable cell culture of a specific cell type and providing a test sample and a control sample thereof, said test sample and said control sample being identical,
(b) Exposing said test sample to a chemical compound in a solvent and exposing said control sample to said solvent for a predetermined period of time,
(c) Determining for the test sample and the control sample gene expressions x i for a subset of i=1 to n genes selected from the group of genes corresponding to SEQ ID NOs 1 to 153,
(d) For this subset of n genes looking up in a database the gene expressions x i for a set of control and test samples, the test samples being exposed for said predetermined period of time to a set of chemical sensitizing model compounds comprising both sensitizers and non-sensitizers,
(e) Using the gene expressions of the said test sample and the said control sample of step (c) as input to a statistical classification model that is based on said database and that is trained and optimized to classify chemical compounds as either sensitizers or non-sensitizers using gene expressions x i for said subset of n genes, and
(f) Predicting through said model whether the chemical compound tested belongs to the class of sensitizers or to the class of non-sensitizers.</p> |
