发明名称 |
KNOCK-IN VECTORS FOR PRODUCING BIOACTIVE SUBSTANCES BY USING PORCINE beta-CASEIN GENOMIC DNA, AND PROCESSES FOR PRODUCING BIOACTIVE SUBSTANCES USING THE SAME |
摘要 |
A knock-in vector for producing a bioactive substance is provided to efficiently and stably express bFGF (basic Fibroblast Growth Factor) and GFP (Green Fluorescent Protein) from porcine milk. A knock-in vector for producing a bioactive substance comprises 5' end fragment, gene coding the bioactive substance, and 3' end fragment. The 5' end fragment is 5' arm and comprises 2.65kb of promoter, one axon and one intron. The 3' end fragment is 3' arm, and comprises an axon 5, 6, and 7 of porcine beta-casein genome DNA. A nuclear localization signal (NLS) is additionally connected to the 5' end. The knock-in vector comprises a positive selectable marker and negative selectable marker. The bioactive substance is basic fibroblast growth factor (bFGF) or green fluorescent protein (GFP).
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申请公布号 |
KR20090073921(A) |
申请公布日期 |
2009.07.03 |
申请号 |
KR20070141992 |
申请日期 |
2007.12.31 |
申请人 |
INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY |
发明人 |
KANG, MAN JONG;KIM, HYE MIN;LEE, SANG MI;PARK, HYO YOUNG |
分类号 |
C12N15/64;C12N15/09;C12N15/12 |
主分类号 |
C12N15/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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