THREE-DIMENSIONAL STRUCTURE OF FABK PROTEIN AND METHOD OF DEVELOPING A FABK PROTEIN INHIBITOR USING THE SAME

摘要

A method for developing a FabK inhibitor is provided to screen a FabK inhibiting agent using active fragment of FabK and three dimensional structure. A method for crystallizing a FabK protein comprises a step of containing FabK protein solution with container solution comprising buffer solution, metal salt and precipitator. The concentration of FabK protein solution is 3-30mg/ml. The pH concentration and concentration is 4-9.5 and 0.005-1.5M. The metal salt is alkaline metal salt and alkaline metal salt. A precipitator is selected from alcohol of C1-4, and the concentration in the container solution is 1-40%(v/v). FabK-FMN(falvin mononucleotide) complex crystalline has three dimensional structure. A method for developing an inhibitor of FabK inhibitor is to use the three dimensional crystalline structure information.