发明名称 |
Cell free methods for detecting protein-ligand binding |
摘要 |
Provided are rapid and sensitive cell-free assay methods for detecting and/or measuring specific bimolecular or higher order interactions via reassembly of a split monomeric reporter protein, and methods of detecting or identifying modulators of such interactions by the effect on the signal provided by the reassembled split reporter protein. This methodology is adaptable to protein-protein, protein-peptide, protein-nucleic acid, protein-methylated or nonmethylated nucleic acid and other small or large molecule ligands and binding proteins.
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申请公布号 |
US2009170069(A1) |
申请公布日期 |
2009.07.02 |
申请号 |
US20080290778 |
申请日期 |
2008.11.03 |
申请人 |
THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA |
发明人 |
GHOSH INDRANEEL;STAINS CLIFF I.;PORTER JASON R.;JESTER BENJAMIN;FURMAN JENNIFER |
分类号 |
C12Q1/70;C12Q1/00;C12Q1/26;C12Q1/34;C12Q1/48;C12Q1/66;G01N33/566 |
主分类号 |
C12Q1/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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