摘要 |
<p>This invention relates to a method of analyzing a biological sample, said sample comprising one or more (poly)peptides, said method comprising (i) bringing said biological sample into contact with a cross-linking fixative; (ii) neutralizing any excess of said cross-linking fixative; (iii) adsorbing the sample fixated in (i) on the surface of a solid support; (iv) adding one or more endoproteases; and (v) detecting of one or more cleavage products of said endoprotease(s) by mass spectrometry. Also provided is a method of processing a biological sample, said sample comprising one or more biological macromolecules, said method comprising exposing the sample to elevated hydrostatic pressure and, either concomitantly and/or subsequently, to a cross-linking fixative. Furthermore provided are kits useful for performing the methods of the invention.</p> |
申请人 |
MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.;LUEHRMANN, REINHARD;KASTNER, BERTHOLD;STARK, HOLGER;URLAUB, HENNING;POERSCHKE, DIETMAR;SANDER, BJOERN;GOLAS, MONIKA;RICHTER, FLORIAN |
发明人 |
LUEHRMANN, REINHARD;KASTNER, BERTHOLD;STARK, HOLGER;URLAUB, HENNING;POERSCHKE, DIETMAR;SANDER, BJOERN;GOLAS, MONIKA;RICHTER, FLORIAN |