发明名称 |
METHOD FOR IDENTIFICATION AND PARENTAGE USING MICROSATELLITE MARKER IN OLIVE FLOUNDER |
摘要 |
<p>An analysis method of genotype of each olive flounder is provided to amplify and analyze eight microsatellite markers of olive flounder and shorten analysis time and expense. A composition which specifically amplifies microsatellite markers of olive flounder of the sequence numbers 1-8 comprises primer pairs of oligonucleotide having sequences 9 and 10, 11 and 12, 13 and 14, 15 and 16, 17 and 18, 19 and 20, 21 and 22, 23 and 24 which specifically amplifies the sequence numbers 1-8. A method for identifying an individual of olive flounder comprises: a step of isolating DNA sample of fish; a step of amplifying the DNA sample using the composition which amplifies the microsatellite marker of olive flounder; and a step of analyzing band pattern of amplified product. A method for identifying parentage of olive flounder comprises: a step of isolating DNA sample of fishes; a step of amplifying the DNA sample; a step of analyzing the band pattern of amplified product; and a step of comparing the band pattern with parental generation fishes.</p> |
申请公布号 |
KR20090069898(A) |
申请公布日期 |
2009.07.01 |
申请号 |
KR20070137720 |
申请日期 |
2007.12.26 |
申请人 |
REPUBLIC OF KOREA |
发明人 |
KIM, KYUNG KIL;KIM, JONG HYUN;LEE, JEONG HO;NOH, JAE KOO;KIM, HYUN CHUL;KIM, WOO JIN;KANG, JUNG HA |
分类号 |
C12Q1/68;C12N15/09;C12N15/10 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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