ANTIBIOTICS MARKER FREE SOYBEAN HAVING RESISTANCE AGAINST TWO HERBICIDES

摘要

An antibiotics marker free soybean has herbicide complex resistance using bar gene and CP4-EPSPS gene is provided to improve the weed prevention effect. A method for manufacturing an antibiotics marker free bean which has herbicide complex resistance comprises: a step of transforming Agrobacterium with a plant expression vector p35S-EPSPS-B which contains bar gene and CP4-EPSPS (CP4 5-enolpyruvylshikimate-3-phosphate synthase) gene but not antibiotics marker gene; a step of co-culturing transformed Agrobacterium and bean; and a step of selecting transformed bean and cultivating.