发明名称 |
METHOD FOR AMPLIFICATION OF NUCLEOTIDE SEQUENCE |
摘要 |
A method for amplification of a nucleotide sequence characterized by performing PCR amplification using mRNA isolated from a biological sample as a template and using a first primer comprising a poly(T) sequence and an additional nucleotide sequence X thereto and a second primer comprising a poly(T) sequence and an additional nucleotide sequence Y thereto, provided that the nucleotide sequences X and Y in the first and second primers, respectively, have different sequences from each other. |
申请公布号 |
EP1845160(A4) |
申请公布日期 |
2009.07.01 |
申请号 |
EP20060713503 |
申请日期 |
2006.02.10 |
申请人 |
RIKEN |
发明人 |
SAITOU, MITINORI;KURIMOTO, KAZUKI;YABUTA, YUKIHIRO;OHINATA, YASUHIDE |
分类号 |
C12N15/09;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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