VECTOR CONSTRUCTION FOR TRANSFORMATION OF BASIDIOMYCETE FUNGUS, POLYPORUS BRUMALIS

摘要

A vector for transforming Polyporus brumalis containing laccase gene which is derived from the Polyporus brumalis is provided to have transformant of white-rotting fungus and produce fungus having enhanced lignin lysis function. A recombination vector for transforming a Polyporus brumalis comprises a liccase gene (sequence No. 9 (SEQ ID NO:9)). The recombination vector further comprises PgpdA promoter (sequence No. 5). The recombinant vector comprises a sequence of the sequence number 10. The recombination vector comprises GFP gene (sequence number 13). The laccase gene is over-expressed by having transformant of white-rotting fungus having enhanced lignin lysis function.