发明名称 |
Recovery of plasmids in an aqueous two-phase system |
摘要 |
The present invention is a method for the purification of plasmid DNA comprising to provide a composition comprising a first polymer having inverse solubility characteristics and a second polymer immiscible in the first polymer; contacting said solution with an aqueous solution comprising plasmid DNA; providing phase separation and isolating the aqueous phase; and increasing the temperature of the isolated phase to a temperature above the cloud point of the first polymer and below the temperature where plasmid DNA is degraded and subsequently isolating the aqueous phase so formed. The invention also encompasses a kit for purification of plasmid DNA as described above.
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申请公布号 |
US7553658(B2) |
申请公布日期 |
2009.06.30 |
申请号 |
US20050526089 |
申请日期 |
2005.02.23 |
申请人 |
GE HEALTHCARE BIO-SCIENCES AB |
发明人 |
KEPKA CECILIA JANSSON;RHODIN EDSO JENNY;TJERNELD FOLKE |
分类号 |
C12N1/08;C12N15/09;C12N15/10;C12N15/64 |
主分类号 |
C12N1/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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