发明名称 |
TRANSGENIC PIG EXPRESSING HLA-G AND DAF AND THE METHOD OF PRODUCING THEREOF |
摘要 |
A method for producing transgenic pig which expresses HLA-G gene and DAF gene is provided to suppresses immune rejection of natural killer cell(NK cell) and use for cell theraphy and xeno-transplantation. A method for producing transgenic pig clone cell line which expresses human leukocyte antigen-G(HLA-G) gene and decay-accelerating factor(DAF) gene comprises: a step of isolating a somatic cell from HLA-G clonal pig; a step of inducing an expression vector containing DAF gene into the somatic cell; and a step of screening somatic cell in which the expression vector is induced. The expression vector is pCX-EGFP-BSD as a cloning vector. A method for producing the transgenic pig which expresses HLA-G gene and DAF gene comprises: a step of culturing the transgenic pig clone somatic cell line; a step of removing a nucleus of ovum and fusing clone somatic cell; and a step of transplanting the ovum to surrogate mother pig.
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申请公布号 |
KR20090068646(A) |
申请公布日期 |
2009.06.29 |
申请号 |
KR20070136343 |
申请日期 |
2007.12.24 |
申请人 |
MGEN INC. |
发明人 |
PARK, KWANG WOOK;SEOL, JAE GOO;CHOI, KI MYUNG;HONG, SEUNG PYO;HAN, GUI SEUNG |
分类号 |
C12N15/12;C12N15/06;C12N15/09 |
主分类号 |
C12N15/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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