COMPLEX OF POLYSACCHARIDE AND DOUBLE-STRANDED RNA

摘要

Disclosed is novel double-stranded RNA having an RNA interference effect, which is improved in the capability of being introduced into a cell and also improved in the resistance against a degradative enzyme and which is not reduced in its RNA interference effect. Specifically disclosed is a complex of a polysaccharide having a ß-1,3-glucan skeleton and double-stranded RNA, which fulfils the following requirements (i) to (iii): (i) the double-stranded RNA has a sense strand comprising a nucleotide sequence complementary to a target sequence in a target gene and an antisense strand comprising a nucleotide sequence complementary to the sense strand, and can inhibit the expression of the target gene; (ii) a single-stranded polydeoxyadenine is bound to the terminal of at least one of the sense strand and the antisense strand of the double-stranded RNA directly or via a linker; and (iii) the polysaccharide and the single-stranded polydeoxyadenine together form a complex. The complex is significantly improved in the capability of being introduced into a cell and the resistance against a degradative enzyme, while keeping its RNA interference effect at an effective level.