NOVEL METHOD OF PREPARING SPECIMEN HAVING EXCELLENT ABILITIES TO MAINTAIN TISSUE MORPHOLOGY AND MAINTAIN NUCLEIC ACID QUALITIES

摘要

Disclosed is a method of preparing a specimen by which both of abilities to maintain tissue morphology and maintain the qualities of a nucleic acid (in particular RNA) can be established. Also disclosed is a method of collecting a desired cell by the microdissection method from a specimen prepared by the preceding method and analyzing gene expression in the desired cell. A method of preparing a specimen from various frozen or unfrozen organs or tissues (excluding hard tissues) of the whole body which comprises the following steps: 1) fixing a target organ or tissue with a PFA solution; and 2) embedding the same in paraffin by the AMeX method.