发明名称 RECOMBINATIONAL CLONING USING NUCLEIC ACID HAVING RECOMBINATION SITE
摘要 PROBLEM TO BE SOLVED: To provide chimeric DNA molecules that have the desired characteristics and/or DNA segments by moving or exchanging segments of a DNA molecule by using the engineered recombination sites and recombination proteins. SOLUTION: Provided are the nucleic acids, vectors and methods for moving or exchanging segments of the nucleic acids. Further provided are the recombinational cloning by the use of nucleic acids, vectors and methods, in vitro and in vivo, for cloning or subcloning the nucleic acid molecule in various vectors. COPYRIGHT: (C)2009,JPO&INPIT
申请公布号 JP2009136297(A) 申请公布日期 2009.06.25
申请号 JP20090022278 申请日期 2009.02.03
申请人 LIFE TECHNOLOGIES CORP 发明人 HARTLEY JAMES L;BRASCH MICHAEL A;TEMPLE GARY F;FOX DONNA K
分类号 C12N15/09;C12N1/21;C12N9/00;C12N15/10;C12N15/64;C12N15/66;C12P19/34;C12Q1/68 主分类号 C12N15/09
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