| 摘要 |
The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure alpha-substituted carboxylic acids, such as, for example, alpha-amino acids and alpha-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.
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