| 摘要 |
It is an objective of the present invention to provide a method of synthesizing a protein having disulfide bonds using a reconstituted protein synthesizing system in a convenient manner with high efficiency. It has been found that a protein having the activity can be obtained and synthesized with good efficiency by using a reconstituted protein synthesizing system comprising purified components, from which enzymes and substrates influencing an oxidation-reduction state are removed and in which a state of redox equilibrium between disulfide and thiol is artificially regulated. Such system is used instead of a cell extract or a crude fraction thereof that contains various enzymes and substrates that maintain a reduced state, such as thioredoxin reductase [EC 1.6.4.5] and glutathion reductase [EC 1.6.4.2], resulting in difficulty in conditioning a redox state.
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