METHODS FOR DETECTING TARGET NUCLEIC ACID USING NUCLEASE ACTIVITY OF NUCLEIC ACID POLYMERASE

摘要

A method for detecting target sequence in nucleic acid molecule sample using the 3'-5' exonuclease activation of nucleic acid polymerase is provided to detect the target sequence with improved specificity and convenience by amplifying the sequence. A method for detecting a target nucleic acid sequence of nucleic acid sample comprises: a step (i) of hybridizing oligonucleotide which a reporter molecule and quencher molecule are bonded with the nucleic acid sample; a step (ii) of reacting a nucleic acid polymerase having the activity of 3'-5' exonuclease with reaction material of the step (i); and a step of detecting specific fluorescence which is changed by the separation of the reporter molecule and quencher molecule in the reaction material of the step (ii).