| 摘要 |
A method for producing a hepatocyte which is derived from an amnion epidermal cell is provided to treat liver diseases through the transplantation of cell and be used as a hepatocyte source for toxicity evaluation. A method for producing a hepatocyte which is derived from an amnion epidermal cell having the functions of albumin synthesis, urea synthesis, and ammonia removement comprises: a step of preparing amnion epidermal cell; a step of culturing the amnion epidermal cell in medium; and a step of differentiating the amnion epidermal cell to hepatocyte in serum medium in which a HGF (hepatocyte growth factor) is added. The serum medium contains mixture medium having DMEM(Dulbecco's Modified Eagle's Medium) and F-12 in a ratio of 3:1, fetal bovine serum, L-glutamine, hydrocortisone, insulin, transferring, epidermal growth factor, cholera toxin, triiodotyronine, and glucagon.
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