CONTAMINATION-FREE REAGENTS FOR NUCLEIC ACID AMPLIFICATION

摘要

Methods and kits for generating contamination-free reagents and reagent solutions for use in nucleic acid amplification are provided. Methods include processing of polymerase solutions, nucleotide solutions and primer solutions to render contaminating nucleic acid inert. The methods employ the proofreading activity of the polymerase and/or exonucleascs to de-contaminate the reagents and reagent solutions. Methods and kits for contamination-free nucleic acid amplification are provided.