| 摘要 |
A method of separating a target molecule or cell of interest from a sample is provided. The method comprising: (a) contacting a sample including the target molecule or cell of interest with: (i) a non-immobilized coordinator ion or molecule; and (ii) a non-immobilized composition which comprises at least one ligand capable of binding directly or indirectly the target molecule or cell of interest, the at least one ligand being attached to at least two coordinating moieties selected capable of directing formation of a non-covalent complex when co-incubated with the non-immobilized coordinator ion or molecule and the target molecule or cell of interest, wherein the contacting is effected in a solution having a predetermined volume; and (b) applying a gravitational or centrifugal force on the solution, in a magnitude and a time period sufficient to concentrate at least 70 % of the non-covalent complex in no more than 10 % of the volume as a suspension, resulting in a solute phase separation between the no more than 10 % of the volume and a remaining of the volume. (c) collecting or disopsing the no more than 10 % of the volume, thereby separating the target molecule or cell of interest from the sample. |
