发明名称 |
ION-SELECTIVE SENSORS |
摘要 |
The application provides ion-selective sensors capable of selectively measuring ions, e.g., Na+, K+, Cl-, etc., in the cytosol of a single living cell. The sensor comprises one or more quantum dots or a fluorescent dye, a pH-sensitive dye, and an ion-selective component such as an ionophore disposed in a polymer matrix. The polymer matrix comprises an internalizing moiety which enables the sensor to localize within the cytosol of a cell. The internalizing moiety comprises a small molecule or peptide such as an amine, antepennepedia, mastoparan, or melittin that react under acidic conditions to release a sensor from the confines of a endosome. Once in the cytosol the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g., fluorescence, from the quantum dot or dye by directly absorbing its fluorescence emission.
|
申请公布号 |
WO2009051703(A8) |
申请公布日期 |
2009.06.11 |
申请号 |
WO2008US11726 |
申请日期 |
2008.10.14 |
申请人 |
THE CHARLES STARK DRAPER LABORATORY, INC.;CLARK, HEATHER, A.;HARJES, DANIEL, I.;DUBACH, JOHN, M. |
发明人 |
CLARK, HEATHER, A.;HARJES, DANIEL, I.;DUBACH, JOHN, M. |
分类号 |
G01N33/58;C12M1/34;G01N21/64;G01N27/333 |
主分类号 |
G01N33/58 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|