TRANSPOSON-MEDIATED MUTAGENESIS IN SPERMATOGONIAL STEM CELLS

摘要

The present invention relates to a method for generating mutations in spermatogonial stem cells comprising introducing at least one transposon into the spermatogonial stem cells. The invention further relates to cells obtainable by the method of the invention, a method for linking physiological data with genetic information and also relates to a transposon. Further, the invention relates to a method of generating non-human animals comprising the mutated spermatogonial stem cells as well as to a non-human animals comprising the mutated spermatogonial stem cell of the invention. Furthermore, the invention relates to a method of saturating a genetic locus with mutations in a spermatogonial stem cell and finally, the invention relates to a spermatogonial stem cell library comprising mutated spermatogonial stem cells of the invention.