发明名称 |
PROCESS FOR PREPARING VARIANT POLYNUCLEOTIDES |
摘要 |
The present invention discloses a process for the preparation of variant polynucleotides using a combination of mutagenesis of a starting population of polynucleotides and recombination of the mutated polynucleotides. The process comprises the steps of subjecting a population of polynucleotides to (a series of) two (or more) separate PCR's comprising a first PCR with a forward mutation-specific primer for a position to be mutated and a reverse universal primer and a second PCR with a forward universal primer and a reverse mutation-specific primer for a position to be mutated. The products of the (series of) two (or more) PCR's are assembled by a polymerase, preferably in one tube. |
申请公布号 |
EP1409667(B2) |
申请公布日期 |
2009.06.10 |
申请号 |
EP20020790188 |
申请日期 |
2002.07.23 |
申请人 |
DSM IP ASSETS B.V. |
发明人 |
BOVENBERG, ROELOF, ARY, LANS;KERKMAN, RICHARD |
分类号 |
C12N15/10;C07K1/00;C12N9/80;C12P13/04;C12P41/00 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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