| 摘要 |
A process for the propagation of Theobroma cacao L. by somatic embryogenesis using explant material which is subjected to
a) primary embryogenesis in the dark in a solid culture medium causing induction and expression to produce primary embryos,
b) secondary embryogenesis
i) in which the primary embryos are treated in the dark in a solid or liquid culture medium to produce and multiply embryogenic callus followed by
ii) treatment of the embryogenic callus in the dark in a suitable liquid culture medium causing expression of the embryogenic callus to produce further new secondary embryos,
c) pre-germination of the secondary embryos in a Petri dish on a solid medium, or in a bioreactor in a liquid medium, into pre-germinated secondary embryos at the cotyledonary stage,
d) ex-vitro germination of the pre-germinated secondary embryos at the cotyledonary stage by sowing directly on a culture substrate in the greenhouse to produce the plantlets, and
e) development of the plantlets. |
