SEPARATION AND DETECTION DEVICE WITH MEANS FOR OPTIMIZATION OF THE CAPILLARY DRAG FORCE

摘要

The present invention relates to a method and a device for separating a suspension into a liquid phase and a retentate phase. The device comprises a separation chamber comprising an application zone and a hydrophilic filter material. The separation chamber is connected to a first capillary channel, said capillary channel comprising a reaction zone and optionally a stop zone, where at least the lower part of the internal surface of the reaction zone facing the liquid is hydrophilic and where this hydrophilic surface is further coated with a coating comprising a hydrophilic substance. This coating increases the capillary drag force of the capillary channel and thereby decreases filling time. The invention further relates to a device and a method for quantitative detecting of the presence or absence of a target analyte in a liquid sample, the device comprising a reaction chamber in the form of a capillary channel having a volume of less than 200µl.