COMPARTMENTALIZED TISSUE CULTURE FLASK

摘要

A cell culture device comprising a container having a cell culture compartment defined by a lower gas permeable film and an upper sheet selectively permeable to compounds of selected sizes and impermeable to cells. The devic e is adapted to allow culture medium to reside between the upper sheet and the lower gas permeable film. A basal medium compartment is located above the upper sheet and is adapted to allow basal medium to reside upon the upper sheet. Each compartme nt contains an access port. A gas film support below and in partial contact wit h the gas permeable film holds the gas permeable film in a substantially horizontal position so that suspension or adherent cells can distribute across the surface of the gas permeable film.