METHOD FOR DETECTION/QUANTIFICATION OF NUCLEIC ACID, AND GENE DETECTION SYSTEM

摘要

PROBLEM TO BE SOLVED: To provide a microarray using NASBA that can make quantitative measurement. SOLUTION: The method for detection/quantification of nucleic acid through a solid phase primer immobilized on a substrate at the 5'-terminal, includes at least (1) synthesizing cDNA using a liquid-phase primer having a promoter sequence and a reverse transcriptase, thereby obtaining a cDNA-RNA complex; (2) degrading the RNA in the complex; (3) synthesizing a double-stranded DNA through the cDNA obtained in the step (2) and the solid-phase primer; (4) synthesizing RNA from the double-stranded DNA; (5) synthesizing cDNA through the RNA obtained in the step (4) and the solid-phase primer, thereby obtaining a cDNA-RNA complex; (6) degrading the RNA in the complex obtained in the step (5); (7) synthesizing double-stranded DNA through the cDNA obtained in the step (6) and the liquid-phase primer; and (8) quantifying the double-stranded DNA obtained in the steps (3) and (7). COPYRIGHT: (C)2009,JPO&INPIT