摘要 |
A sample (103) containing a microorganism is divided into a first sample portion (107) and a second sample portion (113), a growth medium i(109, 114) is added to both sample portions and an antibiotic (110) is combined with the second sample portion. The two portions are incubated and then molecular analysis, such as a polymerase chain reaction, is performed on each of the first and second sample portions to determine the resistance or susceptibility of the microorganism to the antibiotic. The concentration of the microorganism prior to the incubation can be below the detection limit or above the detection limit. If of both the portions test positive for microbe growth, the microbe is resistant. If only the portion with the antibiotic tests negative for growth, then the microbe is susceptible.
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