PURIFICATION METHOD OF HUMAN ERYTHROPOIETIN

摘要

The present invention discloses the a method of purifying human erythropoietin, the method of the present invention comprises the steps of (1) purifying a cell culture or pretreated milk of a transgenic animal by heparin chromatography, (2) purifying a fraction containing human erythropoietin obtained from the step (1) by re versed-phase chromatography, and (3) purifying a fraction containing human erythropoietin obtained from the step (2) by gel filtration chromatography. When human erythropoietin is purified from milk of a transgenic animal, the milk of the transgenic animal is pretreated before performing the step (1), and this pretreatment process comprises the steps of (a) recovering a supernatant from the milk of the transgenic animal by centrifugation, (b) adding a first precipitating agent to the supernatant for first precipitation of impurities, (c) adding a transition metal ion for second precipitation of impurities, and (d) ultrafiltering the supernatant recovered from the step (c).