| 摘要 |
FIELD: medicine. ^ SUBSTANCE: invention refers to medicine, namely to virus inactivation in donor enzyme ceruloplasmin production. The disclosed method involves chromatographic purification of enzyme solution by washing with acetate buffer thereafter followed with elution. The enzyme solution is prepared with a solvent-detergent mixture and pre-washed in two stages while being immobilised on sulphopropylcation sorbent. And the first stage includes application of acetate buffer containing sodium caprilate, sodium chloride and propylene glycol, while the second stage involves acetate buffer containing sodium chloride. ^ EFFECT: invention provides high effectiveness in virus removing and inactivation thereby improving virus safety ceruloplasmin. ^ 9 cl, 6 ex |
